Ighmbp2 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:92862 Grohmann K, et al., Characterization of Ighmbp2 in motor neurons and implications for the pathomechanism in a mouse model of human spinal muscular atrophy with respiratory distress type 1 (SMARD1). Hum Mol Genet. 2004 Sep 15;13(18):2031-42
Assay type:	Western blot
MGI Accession ID:	MGI:3055359
Gene symbol:	Ighmbp2
Gene name:	immunoglobulin mu DNA binding protein 2

Antibody:	anti-Ighmpb2
Detection system:	Secondary antibody coupled to horseradish peroxidase

Results

Image: 1B

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	110 kDa (b)	55 kDa	Genetic Background	Mutant Allele(s)	Sex
wt brain	E15.0 (a)	TS23: brain	Present (c)	Present	involves: C57BL/6 * C57BLKS/J		Not Specified
mut brain	E15.0 (a)	TS23: brain	Not Specified	Present	B6.BKS-Ighmbp2^nmd-2J/J	Ighmbp2^nmd-2J/Ighmbp2^nmd-2J	Not Specified
wt spinal cord	E15.0 (a)	TS23: spinal cord	Present (c)	Present	involves: C57BL/6 * C57BLKS/J		Not Specified
mut spinal cord	E15.0 (a)	TS23: spinal cord	Not Specified	Present	B6.BKS-Ighmbp2^nmd-2J/J	Ighmbp2^nmd-2J/Ighmbp2^nmd-2J	Not Specified
wt muscle	E15.0 (a)	TS23: skeletal muscle	Present (c)	Present	involves: C57BL/6 * C57BLKS/J		Not Specified
mut muscle	E15.0 (a)	TS23: skeletal muscle	Not Specified	Present	B6.BKS-Ighmbp2^nmd-2J/J	Ighmbp2^nmd-2J/Ighmbp2^nmd-2J	Not Specified
wt heart	E15.0 (a)	TS23: heart	Weak	Present	involves: C57BL/6 * C57BLKS/J		Not Specified
mut heart	E15.0 (a)	TS23: heart	Not Specified	Present	B6.BKS-Ighmbp2^nmd-2J/J	Ighmbp2^nmd-2J/Ighmbp2^nmd-2J	Not Specified
wt lung	E15.0 (a)	TS23: lung	Present	Present	involves: C57BL/6 * C57BLKS/J		Not Specified
mut lung	E15.0 (a)	TS23: lung	Not Specified	Present	B6.BKS-Ighmbp2^nmd-2J/J	Ighmbp2^nmd-2J/Ighmbp2^nmd-2J	Not Specified
wt liver	E15.0 (a)	TS23: liver	Weak	Present	involves: C57BL/6 * C57BLKS/J		Not Specified
mut liver	E15.0 (a)	TS23: liver	Not Specified	Present	B6.BKS-Ighmbp2^nmd-2J/J	Ighmbp2^nmd-2J/Ighmbp2^nmd-2J	Not Specified
HEK 293 + HA-Ighmbp2	Control
brain/pre-absorption control	Control
brain/pre-immune control	Control

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) This band represents the full length protein. Authors stated that expression levels were strongly reduced in the mutants.
(c) This was one of the tissues with the highest expression level.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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