Gene Expression Data
Assay Details
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Assay
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Reference:
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J:81355
Yang Y, et al., Wnt5a and Wnt5b exhibit distinct activities in coordinating chondrocyte proliferation and differentiation. Development. 2003 Mar;130(5):1003-15
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2450642
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Gene symbol:
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Ihh
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Gene name:
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Indian hedgehog
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Probe:
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Ihh cDNA1
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
1C:
embryonic day 13.5
(more  )
Specimen
1C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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Specimen
2B, WT:
embryonic day 12.5
(more )
Specimen
2B, WT:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: arm |
Strong |
Regionally restricted |
2B WT Ihh
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Strong expression in developing cartilage
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Specimen
2B, Wnt5a -/-:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Wnt5atm1Amc/Wnt5atm1Amc
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: arm |
Absent |
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2B Wnt5a -/- Ihh
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Expression reduced to below detectable levels in cartilage.
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Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Wnt5atm1Amc/Wnt5atm1Amc
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: arm |
Weak |
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Authors report weak expression in developing cartilage.
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Specimen
2C, WT:
embryonic day 15.5
(more )
Specimen
2C, WT:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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Specimen
2C, Wnt5a -/-:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Wnt5atm1Amc/Wnt5atm1Amc
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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Specimen
5B, WT:
embryonic day 14.5
(more )
Specimen
5B, WT:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: forearm |
Present |
Regionally restricted |
5B WT Ihh
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Expression in prehypertrophic chondrocytes.
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Specimen
6A:
embryonic day 15.5
(more )
Specimen
6A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: humerus |
Present |
Regionally restricted |
6A Ihh
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Expression in prehypertrophic chondrocytes.
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Specimen
6C, WT:
embryonic day 15.5
(more )
Specimen
6C, WT:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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Analysis Tools