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Gene Expression Data
Assay Details
Assay
Reference: J:29279 Patapoutian A, et al., Disruption of the mouse MRF4 gene identifies multiple waves of myogenesis in the myotome. Development. 1995 Oct;121(10):3347-58
Assay type: Immunohistochemistry
MGI Accession ID: MGI:2178649
Gene symbol: Actn2
Gene name: actinin alpha 2
Antibody: anti alpha-actinin (EA-53)
Detection system: Secondary antibody coupled to fluorescein
Assay notes: This antibody is specific for alpha-skeletal and alpha-cardiac muscle actinins and does not react to non-skeletal muscle elements. It is possible that other alpha-actinins are recognized as well.
Results
Specimen 4A: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: trunk somite Present 4A Expression in the somites at the forelimb level.

Specimen 4B: embryonic day 11.0; Myf6tm1Wb/Myf6tm1Wb (more )
Structure Level Pattern Image Note
TS18: trunk dermomyotome Present Regionally restricted 4B Expression in the somites at the forelimb level is reduced to the dorsal, earlier forming myogenic cells.

Specimen 4G: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: trunk somite Present 4G Expression in the somites at the hindlimb level.

Specimen 4H: embryonic day 11.0; Myf6tm1Wb/Myf6tm1Wb (more )
Structure Level Pattern Image Note
TS18: trunk dermomyotome Present Regionally restricted 4H Expression in the somites at the hindlimb level is reduced to the dorsal, earlier forming myogenic cells.

Specimen 6E: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: intercostal muscle Present 6E

Specimen 6F: embryonic day 18.5; Myf6tm1Wb/Myf6tm1Wb (more )
Structure Level Pattern Image Note
TS26: intercostal muscle Present 6F Expression in this mutant is the same as in wild-type animals but shows that these mice have abnormal muscle formation between the ribs.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
08/04/2020
MGI 6.15
The Jackson Laboratory