Gene Expression Data
Assay Details
Assay
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Reference:
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J:29279
Patapoutian A, et al., Disruption of the mouse MRF4 gene identifies multiple waves of myogenesis in the myotome. Development. 1995 Oct;121(10):3347-58
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2178554
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Gene symbol:
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Myog
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Gene name:
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myogenin
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Antibody:
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Anti-Myogenin F5D
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
2B, +/-:
embryonic day 9.0; Myf6tm1Wb/Myf6 +
(more )
Specimen
2B, +/-:
(close )
Genetic Background:
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involves: 129S1/Sv * C57BL/6
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Mutant Allele(s):
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Myf6tm1Wb/Myf6+
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Age:
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embryonic day 9.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2C, -/-:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
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Mutant Allele(s):
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Myf6tm1Wb/Myf6tm1Wb
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Age:
|
embryonic day 9.0
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: trunk somite |
Present |
|
2C
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Expression in caudal somites is the same as in heterozygote embryos.
|
Specimen
4C:
embryonic day 11.0
(more )
Specimen
4C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: trunk somite |
Present |
|
4C
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Expression in the somites at the forelimb level.
|
Specimen
4D:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
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Mutant Allele(s):
|
Myf6tm1Wb/Myf6tm1Wb
|
Age:
|
embryonic day 11.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: trunk somite |
Present |
Regionally restricted |
4D
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Expression in the somites at the forelimb level which expanded ventrally and is restricted compared to wild-type embryos.
|
Specimen
4I:
embryonic day 11.0
(more )
Specimen
4I:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: trunk somite |
Present |
|
4I
|
Expression in the somites at the hindlimb level.
|
Specimen
4J:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
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Mutant Allele(s):
|
Myf6tm1Wb/Myf6tm1Wb
|
Age:
|
embryonic day 11.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS18: trunk dermomyotome |
Present |
Regionally restricted |
4J
|
Expression in the somites at the hindlimb level which is restricted to a few cells of the dorsal myotome.
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