Atoh1em1(flpo)Hlai
Endonuclease-mediated Allele Detail
|
Symbol: |
Atoh1em1(flpo)Hlai |
Name: |
atonal bHLH transcription factor 1; endonuclease-mediated mutation 1, Helen C Lai |
MGI ID: |
MGI:6729714 |
Synonyms: |
Atoh1P2A-FLPo |
Gene: |
Atoh1 Location: Chr6:64706109-64708229 bp, + strand Genetic Position: Chr6, 30.03 cM
|
Alliance: |
Atoh1em1(flpo)Hlai page
|
|
|
Allele Type: |
|
Endonuclease-mediated (Recombinase) |
Mutation: |
|
Insertion
|
|
|
Atoh1em1(flpo)Hlai expression driven by
1 gene
Knock-in expression driven by:
Organism |
Driver Gene |
Note |
mouse |
Atoh1 (MGI:104654) |
|
|
|
|
Mutation details: CRISPR/cas9 genome editing is used to insert a P2A, a FlpO recombinase gene after the last amino acid codon and before the stop codon of Atoh1. The cassette contained a minor mutation at the end of FLPo (the last isoleucine amino acid was changed to a serine), which could have occurred possibly because of an imprecise DNA repair event. But, this amino acid change did not affect the enzymatic function of FLPo.
(J:308450)
|
|
|
Activity: |
Tissue activity of this recombinase allele
|
Driver:
|
Atoh1
(mouse)
|
|
|
|
|
Original: |
J:308450 Ogujiofor OW, et al., An Atoh1 CRE Knock-In Mouse Labels Motor Neurons Involved in Fine Motor Control. eNeuro. 2021 Jan-Feb;8(1):ENEURO.0221-20.2021 |
All: |
1 reference(s) |
|