Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Gt(ROSA)26Sorem1.1(CAG-ERT2/Hoxb8)Sykes
Name:	gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1.1, David Sykes
MGI ID:	MGI:6712697
Synonyms:	HOXER
Gene:	Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance:	Gt(ROSA)26Sorem1.1(CAG-ERT2/Hoxb8)Sykes page

Strain of Origin:

C57BL/6J

Allele Type:    Endonuclease-mediated (Inducible, Inserted expressed sequence) Inducer:    tamoxifen Mutation:    Insertion   Gt(ROSA)26Sorem1.1(CAG-ERT2/Hoxb8)Sykes expresses 1 gene Knock-in expresses: Organism Expressed Gene Note mouse Hoxb8 (MGI:96189)   Mutation details: CRISPR/cas9 genome editing is used to insert a CAG promoter followed by a loxP:STOP:loxP (LSL) followed by the ER-Hoxb8 fusion protein into the Gt(ROSA)26Sor locus. Transient transfection with cre recombinase removes the LSL and leaves a single loxP site. The ER-Hoxb8 fusion protein comprises the hormone binding domain of the human estrogen receptor alpha fused to the murine Hoxb8 sequence. In this system, the ER-Hoxb8 protein is constitutively expressed; the estrogen receptor fusion renders the protein functionally dependent on estrogen. (J:101977)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:	942 strains or lines available

Original:	J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017;
All:	1 reference(s)