Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Prdm9em3Kpgn
Name:	PR domain containing 9; endonuclease-mediated mutation 3, Kenneth Paigen
MGI ID:	MGI:6506065
Synonyms:	Prdm9EK
Gene:	Prdm9  Location: Chr17:15763341-15784616 bp, - strand  Genetic Position: Chr17, 8.95 cM
Alliance:	Prdm9em3Kpgn page

Strain of Origin:

C57BL/6J

Allele Type:    Endonuclease-mediated (Hypomorph) Mutation:    Single point mutation   Mutation details: Using CRISPR/Cas9 technology, a G-to-A substitution (C-to-T on forward strand) was created in glutamic acid codon 360 (initially reported as 365) to change it to a lysine codom (p.E360K). The mutation is located in the PR/SET domain and leads to the loss of most of the enzyme's histone methyltransferase activity. As this allele was created in the C57BL/6 strain, it is based on the Prdm9dom2 allele. (J:280261)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Prdm9 Mutation:	46 strains or lines available

Original:	J:280261 Bhattacharyya T, et al., Prdm9 and Meiotic Cohesin Proteins Cooperatively Promote DNA Double-Strand Break Formation in Mammalian Spermatocytes. Curr Biol. 2019 Mar 18;29(6):1002-1018.e7
All:	1 reference(s)