Prkag2em1Blsg
Endonuclease-mediated Allele Detail
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Symbol: |
Prkag2em1Blsg |
Name: |
protein kinase, AMP-activated, gamma 2 non-catalytic subunit; endonuclease-mediated mutation 1, Bao-Liang Song |
MGI ID: |
MGI:6367663 |
Synonyms: |
H530R-PRKAG2 |
Gene: |
Prkag2 Location: Chr5:25067742-25305640 bp, - strand Genetic Position: Chr5, 11.93 cM
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Alliance: |
Prkag2em1Blsg page
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Allele Type: |
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Endonuclease-mediated (Humanized sequence, Hypomorph) |
Mutation: |
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Single point mutation
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Mutation details: Using CRISPR/Cas9 technology with sgRNA 5'-CTCTTTGCAGGTCCATCGGC-3' and a 121-bp single-stranded oligodeoxynucleotide (ssODN) template, a c.1580A>G point mutation was generated to change histidine codon 527 to an arginine codon (p.His527Arg). This mutation mimics the p.His530Arg mutation found in some human familial Wolff-Parkinson-White (WPW) syndrome patients.
(J:278059)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Prkag2 Mutation: |
53 strains or lines available
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Original: |
J:278059 Xie C, et al., Genome editing with CRISPR/Cas9 in postnatal mice corrects PRKAG2 cardiac syndrome. Cell Res. 2016 Oct;26(10):1099-1111 |
All: |
1 reference(s) |
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