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Prkag2em1Blsg
Endonuclease-mediated Allele Detail
Nomenclature
Symbol: Prkag2em1Blsg
Name: protein kinase, AMP-activated, gamma 2 non-catalytic subunit; endonuclease-mediated mutation 1, Bao-Liang Song
MGI ID: MGI:6367663
Synonyms: H530R-PRKAG2
Gene: Prkag2  Location: Chr5:25067742-25305640 bp, - strand  Genetic Position: Chr5, 11.93 cM
Mutation
origin
Strain of Origin:  C57BL/6
Mutation
description
Allele Type:    Endonuclease-mediated (Humanized sequence, Hypomorph)
Mutation:    Single point mutation
 
Mutation detailsUsing CRISPR/Cas9 technology with sgRNA 5'-CTCTTTGCAGGTCCATCGGC-3' and a 121-bp single-stranded oligodeoxynucleotide (ssODN) template, a c.1580A>G point mutation was generated to change histidine codon 527 to an arginine codon (p.His527Arg). This mutation mimics the p.His530Arg mutation found in some human familial Wolff-Parkinson-White (WPW) syndrome patients. (J:278059)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Prkag2 Mutation:  37 strains or lines available
References
Original:  J:278059 Xie C, et al., Genome editing with CRISPR/Cas9 in postnatal mice corrects PRKAG2 cardiac syndrome. Cell Res. 2016 Oct;26(10):1099-1111
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
05/17/2022
MGI 6.19
The Jackson Laboratory