Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Dbhem1.1(flpo)Rray
Name:	dopamine beta hydroxylase; endonuclease-mediated mutation 1.1, Russell Ray
MGI ID:	MGI:6118955
Synonyms:	DBH-FLPo, Dbhtm1(DBH-FLPo)Rray
Gene:	Dbh  Location: Chr2:27055519-27073216 bp, + strand  Genetic Position: Chr2, 19.29 cM
Alliance:	Dbhem1.1(flpo)Rray page

Germline Transmission:	Earliest citation of germline transmission: J:251840
Parent Cell Line:	AB2.2 (ES Cell)
Strain of Origin:	129S7/SvEvBrd-Hprt1b-m2

Allele Type:    Endonuclease-mediated (Null/knockout, Recombinase) Mutations:    Insertion, Intragenic deletion   Dbhem1.1(flpo)Rray expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Dbh (MGI:94864)   Mutation details: The mouse line was generated by homologous recombination using the CRISPR/Cas9 technology. The coding sequences for optimized FLP recombinase was inserted at the transcriptional start site of the endogenous Dbh gene replacing 106 bps of the first exon; a single lox2722 (modified LoxP) site marks the former position of a floxed neomycin resistance cassette that was deleted by Cre-mediated recombination. The resulting allele is a knock-out of Dbh protein product but express Flpo recombinase under control of the Dbh gene. (J:251840)

Activity:	Tissue activity of this recombinase allele
Driver:	Dbh (mouse) Summary of all recombinase alleles driven by Dbh. Alliance mouse Dbh gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Dbh Mutation:	17 strains or lines available

Original:	J:251840 Sun JJ, et al., Generation of Two Noradrenergic-Specific Dopamine-Beta-Hydroxylase-FLPo Knock-In Mice Using CRISPR/Cas9-Mediated Targeting in Embryonic Stem Cells. PLoS One. 2016;11(7):e0159474
All:	1 reference(s)