Summary | Transgene origin | Transgene description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Tg(CAG-PPARG*,-tdTomato)#Sig
Name:	transgene insertion, Curt D Sigmund
MGI ID:	MGI:5912198
Synonyms:	PPARgamma-P467L
Transgene:	Tg(CAG-PPARG*,-tdTomato)#Sig  Location: unknown
Alliance:	Tg(CAG-PPARG*,-tdTomato)#Sig page

Strain of Origin:

C57BL/6 x SJL

Transgene Type:    Transgenic (Conditional ready, Humanized sequence, Inserted expressed sequence, Reporter) Mutation:    Insertion   Tg(CAG-PPARG*,-tdTomato)#Sig expresses 1 gene Transgene expresses: Organism Expressed Gene Homolog in Mouse Note human PPARG (5468) Pparg  (MGI:97747) Mutated human gene resulting in a Pro467Leu substitution   Mutation details: The transgenic construct comprised the following elements: a CAG promoter, a loxP site flanked STOP cassette, a splicable intron, a mutant human PPARG coding region (from a cDNA) that codes for a substitution resulting in a Pro467Leu change, an IRES in front of the tdTomato reporter gene, a WPRE transcription enhancer and a polyA signal. The construct was injected into the pronuclei of fertilized C57BL/6 x SJL eggs. Only after cre-mediated recombination (which will remove the STOP cassette), will the human and reporter genes be expressed. The mutation in the human gene results in a dominant negative allele. (J:242422)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available

Original:	J:242422 Stump M, et al., Effect of selective expression of dominant-negative PPARgamma in pro-opiomelanocortin neurons on the control of energy balance. Physiol Genomics. 2016 Jul 01;48(7):491-501
All:	1 reference(s)