Summary | Mutation origin | Mutation description | Find Mice (IMSR) | Notes | References

Symbol:	Gt(ROSA)26Sortm12.1Mgn
Name:	gene trap ROSA 26, Philippe Soriano; targeted mutation 12.1, Mark A Magnusson
MGI ID:	MGI:5911670
Synonyms:	Gt(ROSA)26Sortm1(Pdx1,MafA,Neurog3.mCherry)Mgn, Rosa263TF.mCherry, Rosa26(3TF.mCherry)Mgn, Rs263TF.mCherry
Gene:	Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance:	Gt(ROSA)26Sortm12.1Mgn page

Germline Transmission:	Earliest citation of germline transmission: J:241149
Parent Cell Line:	TL1/TL-1 (ES Cell)
Strain of Origin:	129S6/SvEvTac

Allele Type:    Targeted (Inducible, Inserted expressed sequence, RMCE-ready, Reporter) Inducer:    doxycycline/tetracycline Mutation:    Insertion   Gt(ROSA)26Sortm12.1Mgn expresses 3 genes Knock-in expresses: Organism Expressed Gene Note mouse Mafa (MGI:2673307) tetO driven expression of 3 transcription factors mouse Neurog3 (MGI:893591) mouse Pdx1 (MGI:102851)   Mutation details: In mice bearing this allele, doxycycline regulates expression of the mCherry fluorescent reporter and three mouse transcription factors (3TF) - v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian) (encoded in mouse by Mafa), pancreatic and duodenal homeobox 1 (Pdx1), and neurogenin 3 (Neurog3) - in cells expressing either the tetracycline transactivator (tTA) or reverse transactivator (rtTA). The allele was generated by recombinase-mediated cassette exchange (RMCE) in ES cells harboring the cassette acceptor allele Gt(ROSA)26Sortm1Mgn. The insertion, flanked by lox71/lox2272 sites, contains a bi-directional tetracycline regulated promoter (a tetracycline responsive element (TRE), comprising seven copies of the tetracycline operator (tetO), flanked by CMV mini promoters) derived from pTRE-Tight-BI driving expression, in one direction, of mCherry, and in the opposite direction, of a single transcript encoding the 3TF separated by 2A peptides, which mediate ribosome skipping via inhibition of peptide bond formation. Excision of a selection cassette has left a single FRT site downstream of mCherry. (J:241149)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:	942 strains or lines available

Original:	J:241149 Clayton HW, et al., Pancreatic Inflammation Redirects Acinar to beta Cell Reprogramming. Cell Rep. 2016 Nov 15;17(8):2028-2041
All:	1 reference(s)