Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Runx2tm1.1Amc
Name:	runt related transcription factor 2; targeted mutation 1.1, Andrew P McMahon
MGI ID:	MGI:5824224
Synonyms:	Runx2-BioFL, Runx2-Biotin-3xFLAG
Gene:	Runx2  Location: Chr17:44806873-45125518 bp, - strand  Genetic Position: Chr17, 21.33 cM
Alliance:	Runx2tm1.1Amc page

Germline Transmission:	Earliest citation of germline transmission: J:328555
Parent Cell Line:	v6.5 (ES Cell)
Strain of Origin:	(C57BL/6 x 129S4/SvJae)F1

Allele Type:    Targeted (Epitope tag) Mutation:    Insertion   Mutation details: The targeting vector contains a loxP-flanked PGKneo cassette downstream of Biotin-3xFLAG (BioFL) sequence (encoding a biotin recognition motif and 3 copies of the FLAG epitope) fused to the C-terminus of the gene. Cre-mediated recombination removed the floxed neo cassette. Western blotting detected levels of Rnx2-BioFL protein similar to wild-type. (J:328555)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Runx2 Mutation:	42 strains or lines available

Original:	J:328555 Hojo H, et al., Runx2 regulates chromatin accessibility to direct the osteoblast program at neonatal stages. Cell Rep. 2022 Sep 6;40(10):111315
All:	2 reference(s)