Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Bhlha15tm4Skz
Name:	basic helix-loop-helix family, member a15; targeted mutation 4, Stephen F Konieczny
MGI ID:	MGI:5770104
Synonyms:	Mist1lox
Gene:	Bhlha15  Location: Chr5:144127104-144131259 bp, + strand  Genetic Position: Chr5, 83.05 cM, cytoband G2-G3
Alliance:	Bhlha15tm4Skz page

Germline Transmission:	Earliest citation of germline transmission: J:231290
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Allele Type:    Targeted (Conditional ready, No functional change) Mutation:    Insertion   Mutation details: A targeting vector was designed to insert a loxP sites flanking exon 2, containing the entire coding region of the basic helix-loop-helix family, member a15 (Bhlha15) gene. The MIST1 coding region was also engineered to contain an E. coli biotin ligase BirA biotin tag (BT) sequence (MASSLRQILDSQKMEWRSNAGGS) at the N-terminus of the protein where BirA can biotinylate the central lysine residue in the sequence. Additionally, the MIST1 coding region contains a MYC/6x His tag at the C-terminus that can be detected using anti-MYC. A frt-flanked neomycin resistance (neo) cassette was inserted downstream of the floxed sequence. (J:231290)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Bhlha15 Mutation:	15 strains or lines available

Original:	J:231290 Karki A, et al., Silencing Mist1 Gene Expression Is Essential for Recovery from Acute Pancreatitis. PLoS One. 2015;10(12):e0145724
All:	3 reference(s)