Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol: Gata4tm1Sho Name: GATA binding protein 4; targeted mutation 1, Stuart Orkin MGI ID: MGI:2386764 Synonyms: Gata4ki Gene: Gata4  Location: Chr14:63436371-63509141 bp, - strand  Genetic Position: Chr14, 33.24 cM Alliance: Gata4tm1Sho page

Reduced coronary microvasculature, decreased capillary density and increased fibrosis in Gata4tm1.1Wtp/Gata4tm1Sho Tg(Myh6-cre)2182Mds/0 hearts Show the 2 phenotype image(s) involving this allele.

Germline Transmission:	Earliest citation of germline transmission: J:77392
Parent Cell Line:	TL1/TL-1 (ES Cell)
Strain of Origin:	129S6/SvEvTac

Allele Type:    Targeted Mutation:    Nucleotide substitutions   Mutation details: The endogenous gene was targeted with a construct in which codon 217 had been modified by site directed mutagensis to encode glycine rather than valine. The targeted residue (corresponding with Val 205 in the human protein) lies within the N finger domain and is involved in zinc finger protein binding. A floxed neo cassette was inserted in the intron following the exon containing the substitution mutation. Comparision with mice carrying Gata4tm1.1Sho indicated that the intronic neo cassette did not affect gene expression. (J:77392)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:	6 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	9 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gata4 Mutation:	36 strains or lines available

Original:	J:77392 Crispino JD, et al., Proper coronary vascular development and heart morphogenesis depend on interaction of GATA-4 with FOG cofactors. Genes Dev. 2001 Apr 1;15(7):839-44
All:	14 reference(s)