Gt(ROSA)26Sortm2.1Vlcg
Targeted Allele Detail
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Symbol: |
Gt(ROSA)26Sortm2.1Vlcg |
Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 2.1, Velocigene |
MGI ID: |
MGI:5546020 |
Synonyms: |
Gt(ROSA26)Sori1COIN-INV(eGFP) |
Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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Alliance: |
Gt(ROSA)26Sortm2.1Vlcg page
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Allele Type: |
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Targeted (Reporter) |
Mutation: |
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Insertion
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Mutation details: A conditional by inversion (COIN) intron consisting of a lox66_SA-Egpf-polyA_lox71 with a dsRed insertion was inserted in the antisense orientation into intron 1 via homologous recombination, splitting intron 1 into 2 new exons (1,036 and 4,595 bp in length). A 2 bp deletion occurs at the site of insertion. The two lox sites are in reverse complementation orientation, thus cre-mediated recombination will irreversibly invert the inserted sequence. In addition, a neomycin resistance cassette was inserted after the the dsRed. Cre-mediated recombination inverts the EGFP cassette to its functional position.
(J:200671)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Gt(ROSA)26Sor Mutation: |
935 strains or lines available
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Original: |
J:200671 Economides AN, et al., Conditionals by inversion provide a universal method for the generation of conditional alleles. Proc Natl Acad Sci U S A. 2013 Aug 20;110(34):E3179-88 |
All: |
1 reference(s) |
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