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Gt(ROSA)26Sortm2.1Vlcg
Targeted Allele Detail
Summary
Symbol: Gt(ROSA)26Sortm2.1Vlcg
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2.1, Velocigene
MGI ID: MGI:5546020
Synonyms: Gt(ROSA26)Sori1COIN-INV(eGFP)
Gene: Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance: Gt(ROSA)26Sortm2.1Vlcg page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:200671
Parent Cell Line:  F1H4 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6NTac)F1
Mutation
description
Allele Type:    Targeted (Reporter)
Mutation:    Insertion
 
Mutation detailsA conditional by inversion (COIN) intron consisting of a lox66_SA-Egpf-polyA_lox71 with a dsRed insertion was inserted in the antisense orientation into intron 1 via homologous recombination, splitting intron 1 into 2 new exons (1,036 and 4,595 bp in length). A 2 bp deletion occurs at the site of insertion. The two lox sites are in reverse complementation orientation, thus cre-mediated recombination will irreversibly invert the inserted sequence. In addition, a neomycin resistance cassette was inserted after the the dsRed. Cre-mediated recombination inverts the EGFP cassette to its functional position. (J:200671)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  935 strains or lines available
References
Original:  J:200671 Economides AN, et al., Conditionals by inversion provide a universal method for the generation of conditional alleles. Proc Natl Acad Sci U S A. 2013 Aug 20;110(34):E3179-88
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory