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Dicer1tm2.1Vlcg
Targeted Allele Detail
Summary
Symbol: Dicer1tm2.1Vlcg
Name: dicer 1, ribonuclease type III; targeted mutation 2.1, Velocigene
MGI ID: MGI:5544770
Synonyms: Dicer1i6COIN
Gene: Dicer1  Location: Chr12:104654001-104718211 bp, - strand  Genetic Position: Chr12, 54.83 cM, cytoband E-F1
Alliance: Dicer1tm2.1Vlcg page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:200671
Parent Cell Line:  F1H4 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6NTac)F1
Mutation
description
Allele Type:    Targeted (Conditional ready, No functional change)
Mutation:    Insertion
 
Mutation detailsA conditional by inversion (COIN) intron consisting of a lox66_SA-Egpf-polyA_lox71 was inserted in the antisense orientation into intron 6 via homologous recombination, splitting intron 6 into 2 new exons (3,770 and 446 bp in length). The two lox sites are in reverse complementation orientation, thus cre-mediated recombination will irreversibly invert the inserted sequence. In addition, an FRT flanked drug selection cassette (hygromycin- or neomycin-delta-thymidine kinase cassette was inserted after the COIN intron. Flp-mediated recombination removed the drug selection cassette. (J:200671)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 1 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Dicer1 Mutation:  94 strains or lines available
Notes
Generated in F1H4 ES cells containing Dicer1tm1.1Vlcg.
References
Original:  J:200671 Economides AN, et al., Conditionals by inversion provide a universal method for the generation of conditional alleles. Proc Natl Acad Sci U S A. 2013 Aug 20;110(34):E3179-88
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory