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Hand1tm2.1Jcc
Targeted Allele Detail
Summary
Symbol: Hand1tm2.1Jcc
Name: heart and neural crest derivatives expressed 1; targeted mutation 2.1, James C Cross
MGI ID: MGI:5544632
Synonyms: Hand1TH
Gene: Hand1  Location: Chr11:57719539-57722973 bp, - strand  Genetic Position: Chr11, 35.27 cM
Alliance: Hand1tm2.1Jcc page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:202357
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Insertion
 
Mutation detailsA cDNA containing the tethered homodimer sequence followed by a flexible linker was inserted into exon 1 downstream of the start site methionine. Cre-mediated recombination removed the floxed puromycin resistance cassette inserted downstream of exon 1. Western blot analysis confirmed the absence of monomer protein in E10.5 or E8.5 embryos. (J:202357)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 6 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Hand1 Mutation:  14 strains or lines available
Notes
This allele was originally generated in ES cells heterozygous for Hand1tm1Jcc.
References
Original:  J:202357 Hu D, et al., The basic helix-loop-helix transcription factor Hand1 regulates mouse development as a homodimer. Dev Biol. 2013 Oct 15;382(2):470-81
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory