Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Mapkapk2tm1.1Yaff
Name:	MAP kinase-activated protein kinase 2; targeted mutation 1.1, Michael Yaffe
MGI ID:	MGI:5528531
Synonyms:	MK2CV
Gene:	Mapkapk2  Location: Chr1:130981437-131025563 bp, - strand  Genetic Position: Chr1, 56.89 cM, cytoband E4
Alliance:	Mapkapk2tm1.1Yaff page

Germline Transmission:	Earliest citation of germline transmission: J:203686
Parent Cell Line:	v6.5 (ES Cell)
Strain of Origin:	(C57BL/6 x 129S4/SvJae)F1

Allele Type:    Targeted (Conditional ready, No functional change) Mutation:    Insertion   Mutation details: A targeting vector was designed to insert a loxP site upstream of exon 2, and a second loxP site (in reverse complement sequence to the first loxP site) downstream of exon 2 as well as a frt:PGK-neo:frt sequence downstream of the second loxP site. Because inward-facing loxP sequences result in cre-mediated inversion rather than excision, exon 2 can reversibly switch from Mapkap2 expression orientation (MK2<+CV>, CV; Cre-versible) to the null orientation (MK2<-CV>) and back as long as Cre recombinase is active in the cell.FLP recombinase mediated recombination removed the neomycin cassette. (J:203686)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Mapkapk2 Mutation:	36 strains or lines available

Original:	J:203686 Morandell S, et al., A Reversible Gene-Targeting Strategy Identifies Synthetic Lethal Interactions between MK2 and p53 in the DNA Damage Response In Vivo. Cell Rep. 2013 Nov 27;5(4):868-77
All:	3 reference(s)