Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol: Pax6tm2(Pax6_i5a,Pax2)Xzh Name: paired box 6; targeted mutation 2, Xin Zhang MGI ID: MGI:5512910 Synonyms: Pax65a Gene: Pax6  Location: Chr2:105499245-105527709 bp, + strand  Genetic Position: Chr2, 55.31 cM Alliance: Pax6tm2(Pax6_i5a,Pax2)Xzh page

No eye structure is seen in E15.5 homozygous Pax6tm2(Pax6_i5a,Pax2)Xzh or Pax6tm2.1(Pax6_i5a,Pax2)Xzh mutants Show the 2 phenotype image(s) involving this allele.

Germline Transmission:	Earliest citation of germline transmission: J:198381
Parent Cell Line:	Not Specified (ES Cell)
Strain of Origin:	129S6/SvEvTac

Allele Type:    Targeted (Conditional ready, Inserted expressed sequence) Mutations:    Insertion, Intragenic deletion   Pax6tm2(Pax6_i5a,Pax2)Xzh expresses 2 genes Knock-in expresses: Organism Expressed Gene Note Not Specified Pax2 Only expressed after Cre-mediated recombination Not Specified Pax6 The DNA binding of the Pax6 gene has been altered   Mutation details: The coding sequence (exons 4 through 13) was replaced with a loxP site, cDNA encoding an alternatively spliced isoform for Pax6 that contains 14 amino acid inserted within the PD domain that changed its DNA binding specificity (5a), an FRT-flanked neomycin resistance/STOP cassette, loxP site and cDNA encoding Pax2. This allele expressed the 5a isoform of Pax6 (Pax6_i5a). (J:198381)

Generation of the Pax6tm2(Pax6_i5a,Pax2)Xzh and Pax6tm2.1(Pax6_i5a,Pax2)Xzh alleles

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Pax6 Mutation:	90 strains or lines available

Original:	J:198381 Carbe C, et al., An allelic series at the paired box gene 6 (Pax6) locus reveals the functional specificity of Pax genes. J Biol Chem. 2013 Apr 26;288(17):12130-41
All:	1 reference(s)