Summary | Mutation origin | Mutation description | Recombinase activity | Phenotypes | Find Mice (IMSR) | References

Symbol:	Myf5tm1(cre/Esr1*)Trdo
Name:	myogenic factor 5; targeted mutation 1, Thomas A Rando
MGI ID:	MGI:5509335
Synonyms:	Myf5CreER
Gene:	Myf5  Location: Chr10:107318769-107321995 bp, - strand  Genetic Position: Chr10, 55.95 cM
Alliance:	Myf5tm1(cre/Esr1*)Trdo page

Germline Transmission:	Earliest citation of germline transmission: J:199482
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Allele Type:    Targeted (Inducible, Recombinase) Inducer:    tamoxifen Mutation:    Insertion   Myf5tm1(cre/Esr1*)Trdo expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Myf5 (MGI:97252)   Mutation details: A targeting vector was designed to place an internal ribosome entry site (IRES) fused to a CreERTM fusion gene (CreERTM; Cre recombinase fused to a G525R mutant form of the mouse estrogen receptor ligand binding domain) downstream of the stop codon in exon 3 of the Myf5 gene. A neomycin resistance marker, flanked by frt sites, was inserted downstream of the IRES-CreERTM cassette. This construct was electroporated into R1 embryonic stem cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric males were bred with C57BL/6 females. (J:199482)

Activity:	Tissue activity of this recombinase allele
Driver:	Myf5 (mouse) Summary of all recombinase alleles driven by Myf5. Comparative matrix view of recombinase activities Alliance mouse Myf5 gene page

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Myf5 Mutation:	17 strains or lines available

Original:	J:199482 Biressi S, et al., Myf5 expression during fetal myogenesis defines the developmental progenitors of adult satellite cells. Dev Biol. 2013 Jul 15;379(2):195-207
All:	1 reference(s)