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Ttm1(cre/ERT2)Hssk
Targeted Allele Detail
Summary
Symbol: Ttm1(cre/ERT2)Hssk
Name: brachyury, T-box transcription factor T; targeted mutation 1, Hiroshi Sasaki
MGI ID: MGI:5490031
Synonyms: TEGFP, TnEGFP-CreERT2
Gene: T  Location: Chr17:8653255-8661328 bp, + strand  Genetic Position: Chr17, 4.92 cM
Alliance: Ttm1(cre/ERT2)Hssk page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:194054
Parent Cell Line:  TT2 (ES Cell)
Strain of Origin:  (C57BL/6NCrlj x CBA/JNCrlj)F1
Mutation
description
Allele Type:    Targeted (Inducible, Recombinase)
Inducer:    tamoxifen
Mutations:    Insertion, Intragenic deletion
 
Ttm1(cre/ERT2)Hssk expression driven by 1 gene
 
Mutation detailsRecombineering of the BAC clone RP23-99E8 containing the T (brachyury) locus generated a targeting vector designed to replace the stop codon of T and introduce sequences encoding the ERAV 2A peptide, a human histone H2B/EGFP fusion protein (protein is expressed in the cell nucleus), the Thosea asigna virus 2A peptide and cre/ERT2 fusion protein. A self-excising ACN cassette (containing the neo resistance marker) was also included. The vector was electroporated into ES cells for homologous recombination. Chimeras were generated from 3 independent clones (36, 41, and 44) and were bred to C57BL/6J females, with the ACN cassette self-excising during passage through the germline. The line derived from #41 was analyzed further. (J:194054)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: T (mouse)
Summary of all recombinase alleles driven by T.
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 17 assay results
In Structures Affected by this Mutation: 1 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any T Mutation:  60 strains or lines available
References
Original:  J:194054 Imuta Y, et al., Generation of knock-in mice that express nuclear enhanced green fluorescent protein and tamoxifen-inducible Cre recombinase in the notochord from Foxa2 and T loci. Genesis. 2013 Mar;51(3):210-8
All:  9 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory