Ttm1(cre/ERT2)Hssk
Targeted Allele Detail
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Symbol: |
Ttm1(cre/ERT2)Hssk |
Name: |
brachyury, T-box transcription factor T; targeted mutation 1, Hiroshi Sasaki |
MGI ID: |
MGI:5490031 |
Synonyms: |
TEGFP, TnEGFP-CreERT2 |
Gene: |
T Location: Chr17:8653255-8661328 bp, + strand Genetic Position: Chr17, 4.92 cM
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Alliance: |
Ttm1(cre/ERT2)Hssk page
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Allele Type: |
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Targeted (Inducible, Recombinase) |
Inducer: |
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tamoxifen |
Mutations: |
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Insertion, Intragenic deletion
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Ttm1(cre/ERT2)Hssk expression driven by
1 gene
Knock-in expression driven by:
Organism |
Driver Gene |
Note |
mouse |
T (MGI:98472) |
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Mutation details: Recombineering of the BAC clone RP23-99E8 containing the T (brachyury) locus generated a targeting vector designed to replace the stop codon of T and introduce sequences encoding the ERAV 2A peptide, a human histone H2B/EGFP fusion protein (protein is expressed in the cell nucleus), the Thosea asigna virus 2A peptide and cre/ERT2 fusion protein. A self-excising ACN cassette (containing the neo resistance marker) was also included. The vector was electroporated into ES cells for homologous recombination. Chimeras were generated from 3 independent clones (36, 41, and 44) and were bred to C57BL/6J females, with the ACN cassette self-excising during passage through the germline. The line derived from #41 was analyzed further.
(J:194054)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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T
(mouse)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any T Mutation: |
60 strains or lines available
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Original: |
J:194054 Imuta Y, et al., Generation of knock-in mice that express nuclear enhanced green fluorescent protein and tamoxifen-inducible Cre recombinase in the notochord from Foxa2 and T loci. Genesis. 2013 Mar;51(3):210-8 |
All: |
9 reference(s) |
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