Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Atxn10tm2a(EUCOMM)Wtsi
Name:	ataxin 10; targeted mutation 2a, Wellcome Trust Sanger Institute
MGI ID:	MGI:5473219
Gene:	Atxn10  Location: Chr15:85220446-85347413 bp, + strand  Genetic Position: Chr15, 40.32 cM
IMPC:	Atxn10 gene page

Mutant Cell Lines:	EPD0932_1_F08, EPD0932_1_G07, EPD0932_1_H08
Germline Transmission:	Unknown
Parent Cell Line:	JM8.N4 (ES Cell)
Strain of Origin:	C57BL/6N
Project Collection:	EUCOMM

Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter) Mutation:    Insertion     Vector: L1L2_Bact_P   Mutation details: The L1L2_Bact_P cassette was inserted at position 85249039 of Chromosome 15 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 85249829. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 1 line available
Carrying any Atxn10 Mutation:	40 strains or lines available

Original:	J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:	2 reference(s)