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Ptf1atm1a(EUCOMM)Hmgu
Targeted Allele Detail
Nomenclature
Symbol: Ptf1atm1a(EUCOMM)Hmgu
Name: pancreas specific transcription factor, 1a; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
MGI ID: MGI:5472669
Gene: Ptf1a  Location: Chr2:19445663-19447501 bp, + strand  Genetic Position: Chr2, 13.37 cM, cytoband A2
Mutation
origin
Mutant Cell Line:  HEPD0844_2_H08
Germline Transmission:  Unknown
Parent Cell Line:  JM8.N4 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo
 
Mutation detailsThe Ifitm2_intron_L1L2_GT1_LF2A_LacZ_BetactP_neo cassette was inserted at position 19446011 of Chromosome 2 (Build GRCm38) within an endogenous exon, creating an artificial intron that splits the original exon into two artificial exons, where the downstream artificial exon is critical. The cassette is composed of the intron of the Ifitm2 gene containing an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 19447591. The critical artificial exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other EUCOMMTOOLs alleles can be found at http://www.knockoutmouse.org/about/eucommtools/vectors. (J:157065)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 1 line available
Carrying any Ptf1a Mutation:  18 strains or lines available
References
Original:  J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015;
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
01/12/2021
MGI 6.16
The Jackson Laboratory