Rnaseh2atm1a(EUCOMM)Hmgu
Targeted Allele Detail
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Symbol: |
Rnaseh2atm1a(EUCOMM)Hmgu |
Name: |
ribonuclease H2, large subunit; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH |
MGI ID: |
MGI:5472526 |
Gene: |
Rnaseh2a Location: Chr8:85683239-85694498 bp, - strand Genetic Position: Chr8, 41.38 cM, cytoband C3
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IMPC: |
Rnaseh2a gene page |
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Mutant Cell Lines: |
HEPD0853_7_A09, HEPD0853_7_D09, HEPD0853_7_D11, HEPD0853_7_E10, HEPD0853_7_E11, HEPD0853_7_F09, HEPD0853_7_H11 |
Germline Transmission: |
Unknown
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Parent Cell Line: |
JM8.N4 (ES Cell)
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Strain of Origin: |
C57BL/6N
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Project Collection: |
EUCOMM
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Allele Type: |
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Targeted (Conditional ready, Null/knockout, Reporter) |
Mutation: |
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Insertion
Vector: L1L2_GT0_LF2A_LacZ_BetactP_neo
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Mutation details: The L1L2_GT0_LF2A_LacZ_BetactP_neo cassette was inserted at position 85684994 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 85687814. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:157065)
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Original: |
J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015; |
All: |
1 reference(s) |
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