Gt(ROSA)26Sortm2(CAG-hop/EYFP)Imayo
Targeted Allele Detail
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Symbol: |
Gt(ROSA)26Sortm2(CAG-hop/EYFP)Imayo |
Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Itaru Imayoshi |
MGI ID: |
MGI:5449172 |
Synonyms: |
Gt(ROSA)26Sortm1(CAG-eNpHR2/EYFP)Imayo, R26-CAG-LF-eNpHR2-EYFP |
Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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Alliance: |
Gt(ROSA)26Sortm2(CAG-hop/EYFP)Imayo page
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Germline Transmission: |
Earliest citation of germline transmission:
J:190172
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Parent Cell Line: |
TC1/TC-1 (ES Cell)
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Strain of Origin: |
129S6/SvEvTac
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Allele Type: |
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Targeted (Conditional ready, Reporter) |
Mutation: |
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Insertion
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Mutation details: The transgene targeted to the locus contains a splice acceptor flanked puro cassette, CAG promoter, floxed stop cassette, FRT-flanked stop cassette, the halorhodopsin from the halophilic bacterium Natronomas pharaonis modified with an N-terminal signal peptide and a C-terminal endoplasmic reticulum export sequence fused in-frame with an enhanced yellow fluorescent protein (eNpHR2-EYFP), and a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability).
(J:190172)
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Original: |
J:190172 Imayoshi I, et al., Light-induced silencing of neural activity in Rosa26 knock-in mice conditionally expressing the microbial halorhodopsin eNpHR2.0. Neurosci Res. 2013 Jan;75(1):53-8 |
All: |
1 reference(s) |
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