Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Nrastm1.1Emfu
Name:	neuroblastoma ras oncogene; targeted mutation 1.1, Ernst-Martin Fuchtbauer
MGI ID:	MGI:5447943
Synonyms:	LTR, LTR9NS, NrasLTR9S
Gene:	Nras  Location: Chr3:102965643-102975230 bp, + strand  Genetic Position: Chr3, 45.25 cM
Alliance:	Nrastm1.1Emfu page

Germline Transmission:	Earliest citation of germline transmission: J:189834
Parent Cell Line:	CJ7 (ES Cell)
Strain of Origin:	129S1/Sv-Oca2+ Tyr+ Kitl+

Allele Type:    Targeted (Not Applicable) Mutation:    Insertion   Mutation details: A floxed PGk/Tn4 neo cassette and the Akv1-99 murine leukemia virus long terminal repeat were inserted into intron 1 via homologous recombination. The viral long terminal repeat was inserted at the proviral integration site before nucleotide 1062 of the GenBank sequence no. L19607. Cre mediated recombination removed the neo cassette. Q-RT-PCR analysis indicated increased mRNA expression in most tissues in heterozygous and homozygous mice. Western blot analysis confirmed increased protein expression. (J:189834)

Schematic of the Nrastm1.1Emfu allele

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

4 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Nras Mutation:	44 strains or lines available

Original:	J:189834 Lassen LB, et al., Nras overexpression results in granulocytosis, T-cell expansion and early lethality in mice. PLoS One. 2012;7(8):e42216
All:	2 reference(s)