Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Axin2tm1(cre/ERT2)Rnu
Name:	axin 2; targeted mutation 1, Roel Nusse
MGI ID:	MGI:5433373
Synonyms:	Axin2creERT2
Gene:	Axin2  Location: Chr11:108811175-108841609 bp, + strand  Genetic Position: Chr11, 71.83 cM
Alliance:	Axin2tm1(cre/ERT2)Rnu page

Germline Transmission:	Earliest citation of germline transmission: J:186834
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Allele Type:    Targeted (Inducible, Recombinase) Inducer:    tamoxifen Mutations:    Insertion, Intergenic deletion   Axin2tm1(cre/ERT2)Rnu expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Axin2 (MGI:1270862)   Mutation details: A cre/ERT2 sequence (cre recombinase fused to a G400V/M543A/L544A triple mutation ofthe human estrogen receptor ligand binding domain) and an FRT-flanked puromycin resistance cassette were knocked into exon 2 of the Axin2 gene. Transient flp expression in ES cells and crosses to flp-expressing strains were unsuccessful at excising the selection cassette, but its presence does not seem to have negative side effects. (J:74286, J:186834)

Activity:	Tissue activity of this recombinase allele
Driver:	Axin2 (mouse) Summary of all recombinase alleles driven by Axin2. Comparative matrix view of recombinase activities Alliance mouse Axin2 gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Axin2 Mutation:	43 strains or lines available

Original:	J:186834 van Amerongen R, et al., Developmental Stage and Time Dictate the Fate of Wnt/beta-Catenin-Responsive Stem Cells in the Mammary Gland. Cell Stem Cell. 2012 Sep 7;11(3):387-400
All:	50 reference(s)