Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Rnaseh2btm1Hgu
Name:	ribonuclease H2, subunit B; targeted mutation 1, MRC Human Genetics Unit
MGI ID:	MGI:5431529
Synonyms:	Rnaseh2bE202X, Rnaseh2btm1-hgu-A174T,E202X
Gene:	Rnaseh2b  Location: Chr14:62569517-62610445 bp, + strand  Genetic Position: Chr14, 33.19 cM, cytoband C3
Alliance:	Rnaseh2btm1Hgu page

Germline Transmission:	Earliest citation of germline transmission: J:186180
Parent Cell Line:	E14TG2aIV (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Nucleotide substitutions   Mutation details: Exon 7 was replaced with a modified exon 7 in which a G to A point mutation results in the amino acid substitution of threonine for alanine at position 174 (A174T). An additional mutation produces a G to T transversion resulting in a stop codon at position 202 (E202X). A floxed neo cassette was inserted upstream of the modified exon 7. Western blot analysis confirmed the absence of protein expression in E9.5 embryos. (J:186180)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:	6 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Rnaseh2b Mutation:	43 strains or lines available

Original:	J:186180 Reijns MA, et al., Enzymatic removal of ribonucleotides from DNA is essential for mammalian genome integrity and development. Cell. 2012 May 25;149(5):1008-22
All:	1 reference(s)