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Col2a1tm1.1Mcali
Targeted Allele Detail
Summary
Symbol: Col2a1tm1.1Mcali
Name: collagen, type II, alpha 1; targeted mutation 1.1, Audrey McAlinden
MGI ID: MGI:5425899
Synonyms: Col2a1+ex2
Gene: Col2a1  Location: Chr15:97873483-97902525 bp, - strand  Genetic Position: Chr15, 53.97 cM
Alliance: Col2a1tm1.1Mcali page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:184499
Parent Cell Line:  EDJ22 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Mutation
description
Allele Type:    Targeted (Modified isoform(s))
Mutations:    Insertion, Nucleotide substitutions
 
Mutation detailsExon 2 was replaced with an exon 2 in which nucleotide substitutions (ACT GCC AGT Ggtcgta to ACG GCC AGT Ggtaagt) resulted in a silent mutation to produce an HaeIII site and mutate the exon IIa 5' splice site. Cre-mediated recombination removed a floxed neo cassette inserted downstream of the modified exon 2. Mice only express isoform IIa. (J:184499)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Col2a1 Mutation:  68 strains or lines available
References
Original:  J:184499 Lewis R, et al., Disruption of the developmentally-regulated Col2a1 pre-mRNA alternative splicing switch in a transgenic knock-in mouse model. Matrix Biol. 2012 Apr;31(3):214-26
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory