About   Help   FAQ
Hprt1tm214(Ple169-EGFP/cre)Ems
Targeted Allele Detail
Summary
Symbol: Hprt1tm214(Ple169-EGFP/cre)Ems
Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 214, Elizabeth M Simpson
MGI ID: MGI:5303301
Gene: Hprt1  Location: ChrX:52077014-52110536 bp, + strand  Genetic Position: ChrX, 29.31 cM, cytoband A6
Mutation
origin
Mutant Cell Lines:  mEMS947, mEMS950, mEMS1012, mEMS1017
Germline Transmission:  Unknown
Parent Cell Line:  E14TG2a (ES Cell)
Strain of Origin:  129P2/OlaHsd
Project Collection: Pleiades Promoter Project
Mutation
description
Allele Type:    Targeted (Recombinase, Reporter)
Mutation:    Insertion     Vector Type: Not Applicable
 
Hprt1tm214(Ple169-EGFP/cre)Ems expression driven by 1 gene
 
Mutation detailsThe Ple169-EGFP/Cre plasmid (pEMS1093) was created by inserting the Ple169 MiniPromoter (derived from specific promoter/enhancer/regulatory regions of the human pogo transposable element with ZNF domain (POGZ) gene) into the multiple cloning site of the pEMS1302 plasmid vector backbone. This placed the Ple169 MiniPromoter upstream of a minimal F5 mutant-frt site, a Kozak consensus sequence, an EGFP/Cre fusion gene (enhanced green fluorescent protein (EGFP; with mutated TAA to TTA stop) and Cre recombinase gene), a nuclear localization signal, a minimal wildtype frt site, an SV40 early polyA signal, a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm. This Ple169-EGFP/Cre construct was targeted as a single copy knock-in to the Hprtb-m3 mutant locus on the X Chromosome via electroporation into E14TG2a embryonic stem cells. (J:164356)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: POGZ (human)
Summary of all recombinase alleles driven by POGZ.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Hprt1 Mutation:  1272 strains or lines available
References
Original:  J:164356 Portales-Casamar E, et al., A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16589-94
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
03/19/2024
MGI 6.23
The Jackson Laboratory