Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Gfaptm2Ito
Name:	glial fibrillary acidic protein; targeted mutation 2, Shigeyoshi Itohara
MGI ID:	MGI:2384719
Synonyms:	Gfaphm1
Gene:	Gfap  Location: Chr11:102778162-102791368 bp, - strand  Genetic Position: Chr11, 66.48 cM, cytoband D
Alliance:	Gfaptm2Ito page

Germline Transmission:	Earliest citation of germline transmission: J:78341
Parent Cell Line:	E14 (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted Mutations:    Insertion, Intragenic deletion, Nucleotide substitutions   Mutation details: The 5' portion of exon 1 was replaced with corresponding human sequence mutagenized to contain substitution mutations at 3 phosphorylation sites, Thr7, Ser13, and Ser38 (human positions). The incorporation of the specific mutations was verified by sequence analysis as well as by Western blot analysis using antibodies directed toward the phosphorylation sites. Northern blot analysis of total brain RNA showed that transcript levels were unaffected in mutant mice. Immunohistochemical analysis of brain tissue showed that the distribution of protein was conserved in mutant mice. (J:78341)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gfap Mutation:	47 strains or lines available

Original:	J:78341 Takemura M, et al., Protective role of phosphorylation in turnover of glial fibrillary acidic protein in mice. J Neurosci. 2002 Aug 15;22(16):6972-9
All:	1 reference(s)