Apobtm1Zc
Targeted Allele Detail
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| Symbol: |
Apobtm1Zc |
| Name: |
apolipoprotein B; targeted mutation 1, Zhouji Chen |
| MGI ID: |
MGI:2183299 |
| Synonyms: |
Apob27.6 |
| Gene: |
Apob Location: Chr12:8027648-8066835 bp, + strand Genetic Position: Chr12, 3.53 cM
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| Alliance: |
Apobtm1Zc page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:76047
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| Parent Cell Line: |
RW-4 (ES Cell)
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| Strain of Origin: |
129X1/SvJ
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| Allele Type: |
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Targeted (Humanized sequence) |
| Mutations: |
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Insertion, Intragenic deletion
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Mutation details: Deletion of nucleotide 5449 in exon 26 and a loxP-flanked PGK-neo cassette inserted into intron 24 were introduced to the gene via homologous recombination. The single nucleotide deletion should result in a truncated protein similar to the apoB-38.9 mutation found in human familial hypobetalipoproteinemia (FHBL). Western blot analysis of homozygous mutants showed an unexpected mutant protein similar to apoB-27.6, a smaller form of mutant protein found in FHBL. Southern blot analysis of homozygous mutant animals showed the gene was correctly targeted.
(J:76047)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Apob Mutation: |
221 strains or lines available
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| Original: |
J:76047 Chen Z, et al., Hypobetalipoproteinemic Mice with a Targeted Apolipoprotein (Apo) B-27.6-specifying Mutation. IN VIVO EVIDENCE FOR AN IMPORTANT ROLE OF AMINO ACIDS 1254-1744 OF ApoB IN LIPID TRANSPORT AND METABOLISM OF THE ApoB-CONTAINING LIPOPROTEIN. J Biol Chem. 2002 Apr 19;277(16):14135-45 |
| All: |
1 reference(s) |
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Analysis Tools
