Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Vcantm1.1Dzim
Name:	versican; targeted mutation 1.1, Dieter R Zimmermann
MGI ID:	MGI:3850559
Synonyms:	VCAN(tm1Zim), VersicanV0/V2-
Gene:	Vcan  Location: Chr13:89803431-89890628 bp, - strand  Genetic Position: Chr13, 45.5 cM
Alliance:	Vcantm1.1Dzim page

Germline Transmission:	Earliest citation of germline transmission: J:150124
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Allele Type:    Targeted (Null/knockout) Mutation:    Insertion   Mutation details: An endoplasmic reticulum retention signal (KDEL) followed by a STOP codon and floxed neo cassette was inserted into exon 7 after codon E749. This exon encodes the GAG-alpha domain. Germ line, cre mediated recombination removed the neo cassette. Quantitative RT-PCR on brain extracts confirmed a 90% reduction in isoform V2 transcript expression and a decline of isoform V0 transcript to 20% (at P0) or 50% (at P30 and P100) of the levels in wild-type mice. Transcript expression of isoform V1 and V3 is unaffected. Immunohistochemistry confirmed the absence of protein expression of isoforms V0 and V2. (J:150124)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Vcan Mutation:	164 strains or lines available

Original:	J:150124 Dours-Zimmermann MT, et al., Versican V2 assembles the extracellular matrix surrounding the nodes of ranvier in the CNS. J Neurosci. 2009 Jun 17;29(24):7731-42
All:	5 reference(s)