Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Mecomtm1.1Miku
Name:	MDS1 and EVI1 complex locus; targeted mutation 1.1, Mineo Kurokawa
MGI ID:	MGI:3849414
Synonyms:	Evi-1-
Gene:	Mecom  Location: Chr3:30005445-30563937 bp, - strand  Genetic Position: Chr3, 12.66 cM
Alliance:	Mecomtm1.1Miku page

Germline Transmission:	Earliest citation of germline transmission: J:149794
Parent Cell Line:	TT2 (ES Cell)
Strain of Origin:	(C57BL/6NCrlj x CBA/JNCrlj)F1

Allele Type:    Targeted (Null/knockout) Mutation:    Intragenic deletion   Mutation details: A loxP site was inserted upstream of exon 4 and a floxed neomycin cassette was placed downstream. After targeting and selection, transient expression of cre recombinase removed the neomycin cassette and exon 4. Exon 4 encodes a zinc finger domain common to the three major protein isoforms of the gene locus. Immunoblot analysis of homozygote embryonic fibroblasts indicated all three isoforms of the gene locus were absent. (J:149794)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:	4 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Mecom Mutation:	81 strains or lines available

Original:	J:149794 Goyama S, et al., Evi-1 is a critical regulator for hematopoietic stem cells and transformed leukemic cells. Cell Stem Cell. 2008 Aug 7;3(2):207-20
All:	8 reference(s)