Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Mapkapk2tm1Mgl
Name:	MAP kinase-activated protein kinase 2; targeted mutation 1, Matthias Gaestel
MGI ID:	MGI:2179724
Synonyms:	MK2-, MK2 KO
Gene:	Mapkapk2  Location: Chr1:130981437-131025563 bp, - strand  Genetic Position: Chr1, 56.89 cM, cytoband E4
Alliance:	Mapkapk2tm1Mgl page

Germline Transmission:	Earliest citation of germline transmission: J:59678
Parent Cell Line:	E14.1 (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: The catalytic domain of this gene, encompassing subdomains V and VI, was replaced with a neomycin resistance cassette via homologous recombination which introduced stop codons in all 3 reading frames and results in a truncated kinase lacking amino acids 130-383. Northern blot of spleen from homozygous mutant animals verified the absence of gene expression and kinase assay of mouse embryonic fibroblasts verified the absence of enzyme activity. (J:59678)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

3 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Mapkapk2 Mutation:	36 strains or lines available

Original:	J:59678 Kotlyarov A, et al., MAPKAP kinase 2 is essential for LPS-induced TNF-alpha biosynthesis [see comments]. Nat Cell Biol. 1999 Jun;1(2):94-7
All:	70 reference(s)