Gt(ROSA)26Sortm2Bgh
Targeted Allele Detail
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Symbol: |
Gt(ROSA)26Sortm2Bgh |
Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Bernhard G Herrmann |
MGI ID: |
MGI:5049881 |
Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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Germline Transmission: |
Unknown
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Parent Cell Line: |
Other (see notes) (ES Cell)
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Strain of Origin: |
Not Specified
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Allele Type: |
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Targeted |
Mutation: |
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Insertion
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Mutation details: An SV40 splice acceptor is fused to a murinized rtTA followed, in order, by the SV40 polyadenylation signal, the 1.2 kb chicken beta-globin insulator, a PGK-hygromycin selection cassette flanked by a loxp and a lox5171 sequence, a promoterless neomycin resistance gene, and, finally, another chicken beta-globin insulator. This insertion is designed so that the floxed hygromycin selection cassette can be replaced by an exchange cassette containing a tetO regulated expression construct and a PGK promoter. Successful exchange places the promoterless neomycin of this insert under the control of the PGK promoter of the exchange cassette. Exchange has been demonstrated to occur as intended in ES cells.
(J:173434)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Gt(ROSA)26Sor Mutation: |
935 strains or lines available
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ES cell lines = C57BL/6 and G4.
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Original: |
J:173434 Vidigal JA, et al., An inducible RNA interference system for the functional dissection of mouse embryogenesis. Nucleic Acids Res. 2010 Jun 1;38(11):e122 |
All: |
1 reference(s) |
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