Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Rxratm1.1Pcn
Name:	retinoid X receptor alpha; targeted mutation 1.1, Pierre Chambon
MGI ID:	MGI:3839151
Synonyms:	Rxraafo
Gene:	Rxra  Location: Chr2:27566452-27652969 bp, + strand  Genetic Position: Chr2, 19.38 cM
Alliance:	Rxratm1.1Pcn page

Germline Transmission:	Earliest citation of germline transmission: J:146761
Parent Cell Line:	H1 (ES Cell)
Strain of Origin:	129S2/SvPas

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: Most of exon 2, all of intron 2 and 102 bp of exon 3, encoding for amino acids 11-132, were deleted in an ES cell line that carried Rxratm1Ipc . A floxed hygro cassette (between exons 2 and 4) and a floxed neo cassette (between exons 9 and 10) were removed by Cre mediated recombination. The final mutant allele lacks both transcriptional activating function 1 and 2. Northern and Western blot analysis confirmed the expression of a truncated mRNA and protein in homozygous and heterozygous embryos. Expression levels of both the truncated mRNA and protein are lower than those for the wild-type mRNA and protein. (J:146761)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:	6 assay results
In Structures Affected by this Mutation:	11 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Rxra Mutation:	30 strains or lines available

Original:	J:146761 Mascrez B, et al., A transcriptionally silent RXRalpha supports early embryonic morphogenesis and heart development. Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4272-7
All:	1 reference(s)