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Lgr4tm1a(EUCOMM)Hmgu
Targeted Allele Detail
Summary
Symbol: Lgr4tm1a(EUCOMM)Hmgu
Name: leucine-rich repeat-containing G protein-coupled receptor 4; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
MGI ID: MGI:5008817
Gene: Lgr4  Location: Chr2:109747992-109844602 bp, + strand  Genetic Position: Chr2, 56.65 cM
Alliance: Lgr4tm1a(EUCOMM)Hmgu page
IMPC: Lgr4 gene page
Mutation
origin
Mutant Cell Lines:  HEPD0729_3_A08, HEPD0729_3_B05, HEPD0729_3_B06, HEPD0729_3_C05, HEPD0729_3_C06, HEPD0729_3_C07, HEPD0729_3_D07, HEPD0729_3_F07, HEPD0729_3_G06, HEPD0729_3_G08, HEPD0729_3_H08
Germline Transmission:  Earliest citation of germline transmission: J:278480
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 109837067 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 109838661. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065, J:278480)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 1 line available
Carrying any Lgr4 Mutation:  94 strains or lines available
References
Original:  J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory