Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol: Gad1tm1Oba Name: glutamate decarboxylase 1; targeted mutation 1, Kunihiko Obata MGI ID: MGI:2177565 Synonyms: GAD67 - Gene: Gad1  Location: Chr2:70391644-70432358 bp, + strand  Genetic Position: Chr2, 41.63 cM, cytoband D Alliance: Gad1tm1Oba page

Gad1tm1Oba/Gad1tm1Oba mice and cleft palate Show the 1 phenotype image(s) involving this allele.

Germline Transmission:	Earliest citation of germline transmission: J:40978
Parent Cell Line:	TT2 (ES Cell)
Strain of Origin:	(C57BL/6NCrlj x CBA/JNCrlj)F1

Allele Type:    Targeted (Null/knockout, Reporter) Mutation:    Insertion   Mutation details: A lacZ gene followed by a neomycin resistance cassette was fused in frame in exon 1 of the gene. RT-PCR analysis on brain RNA derived from homozygous mice demonstrated that no detectable transcript was produced from this allele. Western blot analysis on lysates derived from neonatal brain confirmed that no GAD1 protein is expressed from this allele. (J:40978)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:	14 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gad1 Mutation:	64 strains or lines available

Original:	J:40978 Asada H, et al., Cleft palate and decreased brain gamma-aminobutyric acid in mice lacking the 67-kDa isoform of glutamic acid decarboxylase. Proc Natl Acad Sci U S A. 1997 Jun 10;94(12):6496-9
All:	5 reference(s)