Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Cacna2d3tm1a(KOMP)Wtsi
Name:	calcium channel, voltage-dependent, alpha2/delta subunit 3; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID:	MGI:4942021
Gene:	Cacna2d3  Location: Chr14:28626900-29443821 bp, - strand  Genetic Position: Chr14, 16.8 cM, cytoband B
Alliance:	Cacna2d3tm1a(KOMP)Wtsi page
IMPC:	Cacna2d3 gene page

Mutant Cell Lines:	EPD0741_1_F11, EPD0741_1_G11, EPD0796_1_A10, EPD0796_1_B11, EPD0796_1_C09, EPD0796_1_H10
Germline Transmission:	Earliest citation of germline transmission: J:188991
Parent Cell Line:	JM8A3.N1 (ES Cell)
Strain of Origin:	C57BL/6N-Atm1Brd
Project Collection:	KOMP-CSD

Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter) Mutation:    Insertion     Vector: L1L2_Bact_P   Mutation details: The L1L2_Bact_P cassette was inserted at position 29068675 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 29069591. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 1 line available
Carrying any Cacna2d3 Mutation:	64 strains or lines available

Original:	J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009;
All:	3 reference(s)