Ewsr1tm1(NCOM)Mfgc
Targeted Allele Detail
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| Symbol: |
Ewsr1tm1(NCOM)Mfgc |
| Name: |
Ewing sarcoma breakpoint region 1; targeted mutation 1, Mammalian Functional Genomics Centre |
| MGI ID: |
MGI:4880180 |
| Gene: |
Ewsr1 Location: Chr11:5019689-5049266 bp, - strand Genetic Position: Chr11, 3.22 cM, cytoband A1-A3
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| IMPC: |
Ewsr1 gene page |
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| Mutant Cell Line: |
N00080P0_C_48W_D3 |
| Germline Transmission: |
Unknown
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| Parent Cell Line: |
C2 (Tcp) (ES Cell)
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| Strain of Origin: |
C57BL/6N
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| Project Collection: |
NorCOMM
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| Allele Type: |
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Targeted (Null/knockout, Reporter) |
| Mutations: |
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Insertion, Intragenic deletion
Vector: L1L2_NTARU-0
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Mutation details: The insertion of NorCOMM cassette pNTARU created a deletion of size 4744bp between positions 5037702-5042446 of Chromosome 11 (Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an F3 site followed by a universal inverse PCR primer site and a splice acceptor lacZ-neomycin phosphotransferase fusion (beta-geo) sequence. This is followed by a PhiC31 attP site and a puromycin sequence that is not active unless a promoter is inserted by PhiC31 at the attP site. The cassette is finished with an FRT site. Variations of this allele can be made using PhiC31 docking and subsequent cloning (Nagy et al., 2009. Methods Mol. Biol. 530:365-78).
(J:165964)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Ewsr1 Mutation: |
92 strains or lines available
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| Original: |
J:165964 Mammalian Functional Genomics Centre, Alleles produced for the NorCOMM project by the Mammalian Functional Genomics Centre (Mfgc), University of Manitoba. MGI Direct Data Submission. 2010; |
| All: |
1 reference(s) |
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Analysis Tools
