Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Tal1tm1Por
Name:	T cell acute lymphocytic leukemia 1; targeted mutation 1, Catherine Porcher
MGI ID:	MGI:3808120
Synonyms:	sclRER
Gene:	Tal1  Location: Chr4:114913623-114928952 bp, + strand  Genetic Position: Chr4, 52.73 cM
Alliance:	Tal1tm1Por page

Germline Transmission:	Earliest citation of germline transmission: J:139179
Parent Cell Line:	E14TG2a (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted Mutation:    Nucleotide substitutions   Mutation details: Three highly conserved DNA-binding residues encoded in exon 6 (Arg-Glu-Arg at positions 195-197) were mutated to alanine residues. A floxed neo cassette was also inserted upstream of exon 6. Transient expression of Cre recombinase excised the neo cassette leaving behind a loxP site. The mutant protein was expressed at endogenous levels in the fetal liver, was localized to cellular nuclei, but lacked the ability to bind DNA. (J:139179)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

4 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Tal1 Mutation:	33 strains or lines available

Original:	J:139179 Kassouf MT, et al., Differential use of SCL/TAL-1 DNA-binding domain in developmental hematopoiesis. Blood. 2008 Aug 15;112(4):1056-67
All:	1 reference(s)