Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Axin2tm1a(KOMP)Wtsi
Name:	axin 2; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID:	MGI:4841043
Gene:	Axin2  Location: Chr11:108811175-108841609 bp, + strand  Genetic Position: Chr11, 71.83 cM
Alliance:	Axin2tm1a(KOMP)Wtsi page
IMPC:	Axin2 gene page

Mutant Cell Lines:	EPD0685_5_F12, EPD0685_5_G09
Germline Transmission:	Earliest citation of germline transmission: J:204812
Parent Cell Line:	JM8A3.N1 (ES Cell)
Strain of Origin:	C57BL/6N-Atm1Brd
Project Collection:	KOMP-CSD

Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter) Mutation:    Insertion     Vector: L1L2_Pgk_P   Mutation details: The L1L2_Pgk_P cassette was inserted at position 108829682 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 108830463. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 1 line available
Carrying any Axin2 Mutation:	43 strains or lines available

Original:	J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009;
All:	3 reference(s)