Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Apptm2.1Zhe
Name:	amyloid beta precursor protein; targeted mutation 2.1, Hui Zheng
MGI ID:	MGI:4838049
Synonyms:	App sbeta KI
Gene:	App  Location: Chr16:84751236-84972187 bp, - strand  Genetic Position: Chr16, 46.92 cM, cytoband C3-qter
Alliance:	Apptm2.1Zhe page

Germline Transmission:	Earliest citation of germline transmission: J:165417
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Allele Type:    Targeted Mutation:    Insertion   Mutation details: The mutated allele contains a loxP site immediately upstream of an exon 16 sequence modified by the insertion of a FLAG motif and a stop codon just downstream of the beta secretase cleavage site. The mutated copy of exon 16 was separated from a second, intact, copy of exon 16 by a sequence cassette containing two copies of neomycin flanked by FRT sites and followed by a second loxP site. Flp recombinase was then used to excise the two neomycin sequences leaving the mutated exon 16 surrounded by loxP sites. Transmembrane and intracellular sequences are deleted from the translation product. (J:165417)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any App Mutation:	89 strains or lines available

Original:	J:165417 Li H, et al., Soluble amyloid precursor protein (APP) regulates transthyretin and Klotho gene expression without rescuing the essential function of APP. Proc Natl Acad Sci U S A. 2010 Oct 5;107(40):17362-7
All:	2 reference(s)