Hprt1<tm34(Ple186-EGFP)Ems> Targeted Allele Detail MGI Mouse (MGI:4834787)

Hprt1^{tm34(Ple186-EGFP)Ems}
Targeted Allele Detail

Summary

Symbol:	Hprt1^{tm34(Ple186-EGFP)Ems}
Name:	hypoxanthine phosphoribosyltransferase 1; targeted mutation 34, Elizabeth M Simpson
MGI ID:	MGI:4834787
Synonyms:	Hprt^{tm34(mEMS2748)Ems}, Ple186, S100B-D-EGFP
Gene:	Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
Alliance:	Hprt1^{tm34(Ple186-EGFP)Ems} page

Mutation
origin

Mutant Cell Lines:	mEMS2748, mEMS2752, mEMS2759, mEMS2761
Germline Transmission:	Earliest citation of germline transmission: J:145689
Parent Cell Line:	mEMS1204 (ES Cell)
Strain of Origin:	(B6.129P2-Hprt1^b-m3/J x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1
Project Collection:	Pleiades Promoter Project

Mutation
description

Allele Type:		Targeted (Reporter)
Mutation:		Insertion Vector Type: Not Applicable
		Mutation details: The Ple186-EGFP transgene (pEMS1385) was designed with the 3980 bp Ple186 minipromoter (S100b-D;derived from a subsection of the promoter and a downstream putative regulatory element from the human S100 calcium binding protein B (S100B) gene) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtsup>b-m3 mutant locus on the X chromosome. The promoter/regulatory regions of the human S100 calcium binding protein B (S100B) gene directs expression of enhanced green fluorescent protein (EGFP) to Bergmann glial cells of the cerebellum. Purkinje cells show high background staining. No EGFP signal above background level could be observed in other regions of the brain, such as cortex and brainstem. (J:144244, J:164356)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Hprt1 Mutation:	1273 strains or lines available

Notes

Germ line transmission of mutant cell line mEMS2748 has been established.

References

Original:	J:145689 The Pleiades Promoter Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2009;
All:	3 reference(s)

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