Hprt1<tm32(Ple112-EGFP)Ems> Targeted Allele Detail MGI Mouse (MGI:4834653)

Hprt1^{tm32(Ple112-EGFP)Ems}
Targeted Allele Detail

Summary

Symbol:	Hprt1^{tm32(Ple112-EGFP)Ems}
Name:	hypoxanthine phosphoribosyltransferase 1; targeted mutation 32, Elizabeth M Simpson
MGI ID:	MGI:4834653
Synonyms:	HCRT-EGFP, Hprt^{tm32(mEMS1736)Ems}, Ple112
Gene:	Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
Alliance:	Hprt1^{tm32(Ple112-EGFP)Ems} page

Mutation
origin

Mutant Cell Lines:	mEMS1736, mEMS1737, mEMS1746
Germline Transmission:	Earliest citation of germline transmission: J:145689
Parent Cell Line:	mEMS1204 (ES Cell)
Strain of Origin:	(B6.129P2-Hprt1^b-m3/J x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1
Project Collection:	Pleiades Promoter Project

Mutation
description

Allele Type:		Targeted (Reporter)
Mutation:		Insertion Vector Type: Not Applicable
		Mutation details: The Ple112-EGFP transgene (pEMS1418) was designed with the 1295 bp Ple112 minipromoter (HCRT-B; derived from a subsection of the promoter and an upstream putative regulatory element from the human hypocretin (orexin) neuropeptide precursor (HCRT) gene) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt^b-m3 mutant locus on the X chromosome. The promoter/regulatory regions of the human hypocretin (HCRT) gene directs expression of enhanced green fluorescent protein (EGFP) to the hypothalamus. Under the higher magnification, EGFP immunoreactivity can be detected in the cell bodies and processes of hypothalamic neurons. Other (lateral) regions of brain did not display any EGFP-positive cells. (J:144244, J:164356)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Hprt1 Mutation:	1273 strains or lines available

Notes

Germ line transmission of mutant cell line mEMS1736 has been established.

References

Original:	J:145689 The Pleiades Promoter Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2009;
All:	3 reference(s)

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