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Gt(ROSA)26Sortm32.1(CAG-COP4*H134R/EYFP)Hze
Targeted Allele Detail
Nomenclature
Symbol: Gt(ROSA)26Sortm32.1(CAG-COP4*H134R/EYFP)Hze
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 32.1, Hongkui Zeng
MGI ID: MGI:4834652
Synonyms: Ai32DeltaNeo, Ai32D (Rosa-CAG-LSL-ChR2(H134R)-EYFP-WPRE), Rosa26-LSL-ChR2-YFP
Gene: Gt(ROSA)26Sor  Location: Chr6:113067428-113077244 bp, - strand  Genetic Position: Chr6, 52.73 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:155793
Parent Cell Line:  G4 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6NCrl)F1
Mutation
description
Allele Type:    Targeted (Inserted expressed sequence, Reporter)
Mutation:    Insertion
 
Mutation detailsThe Rosa-CAG-LSL-ChR2(H134R)-EYFP-WPRE targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), a ChR2(H134R)-EYFP fusion gene, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a BGH polyA signal, and an attB/attP-flanked PGK-FRT-Neo-polyA cassette. To create the ChR2(H134R)-EYFP fusion gene, a cDNA sequence encoding the first 315 amino acids of channelrhodopsin-2 (derived from the green alga Chlamydomonas reinhardtii) was modified with a gain-of-function H134R substitution (CAC to CGC) designed to cause larger stationary photocurrents. This ChR2(H134R) sequence was fused in-frame to the amino terminus of an enhanced yellow fluorescent protein sequence, resulting in the final ChR2(H134R)-EYFP fusion protein sequence. This entire construct was inserted between exons 1 and 2 of the locus. PhiC31 mediated recombination removed the PGK-FRT-Neo-polyA cassette. (J:155793)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  501 strains or lines available
References
Original:  J:155793 Madisen L, et al., A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Nat Neurosci. 2010 Jan;13(1):133-40
All:  9 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
10/01/2019
MGI 6.14
The Jackson Laboratory