Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Notch1tm1a(EUCOMM)Hmgu
Name:	notch 1; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
MGI ID:	MGI:4455755
Gene:	Notch1  Location: Chr2:26347914-26393834 bp, - strand  Genetic Position: Chr2, 18.91 cM
Alliance:	Notch1tm1a(EUCOMM)Hmgu page
IMPC:	Notch1 gene page

Mutant Cell Lines:	HEPD0627_7_A08, HEPD0627_7_B09, HEPD0627_7_B12, HEPD0627_7_D08, HEPD0627_7_D09, HEPD0627_7_D11, HEPD0627_7_E11, HEPD0627_7_F07, HEPD0627_7_F08, HEPD0627_7_F09, HEPD0627_7_F11, HEPD0627_7_F12, HEPD0627_7_H09, HEPD0627_7_H10, HEPD0627_7_H12
Germline Transmission:	Earliest citation of germline transmission: J:204812
Parent Cell Line:	JM8A3.N1 (ES Cell)
Strain of Origin:	C57BL/6N-Atm1Brd
Project Collection:	EUCOMM

Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter) Mutation:    Insertion     Vector: L1L2_Bact_P   Mutation details: The L1L2_Bact_P cassette was inserted at position 26391612 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 26392281. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 3 strains available      Cell Lines: 1 line available
Carrying any Notch1 Mutation:	115 strains or lines available

Original:	J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015;
All:	2 reference(s)