Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Mdm2tm1a(EUCOMM)Wtsi
Name:	transformed mouse 3T3 cell double minute 2; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID:	MGI:4451970
Gene:	Mdm2  Location: Chr10:117524780-117546663 bp, - strand  Genetic Position: Chr10, 66.32 cM, cytoband C1-C3
IMPC:	Mdm2 gene page

Mutant Cell Lines:	EPD0521_5_A02, EPD0521_5_B02, EPD0521_5_B03, EPD0521_5_C03, EPD0521_5_E01, EPD0521_5_E02, EPD0521_5_F01, EPD0521_5_F02, EPD0521_5_G02, EPD0521_5_G03, EPD0521_5_H02
Germline Transmission:	Unknown
Parent Cell Line:	JM8A3.N1 (ES Cell)
Strain of Origin:	C57BL/6N-Atm1Brd
Project Collection:	EUCOMM

Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter) Mutation:    Insertion     Vector: L1L2_gt1   Mutation details: The L1L2_gt1 cassette was inserted at position 117530571 of Chromosome 10 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 117531412. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 1 line available
Carrying any Mdm2 Mutation:	54 strains or lines available

Original:	J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:	2 reference(s)