Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	H2-Ab1b-tm1.1Sish
Name:	histocompatibility 2, class II antigen A, beta 1; targeted mutation 1.1, Satoshi Ishido
MGI ID:	MGI:4436864
Synonyms:	H2-Ab1tm1.1Sish, I-AbetaK225R, MHC-II I-Ab K225R-knockin
Gene:	H2-Ab1  Location: Chr17:34482201-34488392 bp, + strand  Genetic Position: Chr17, 17.98 cM
Alliance:	H2-Ab1b-tm1.1Sish page

Germline Transmission:	Earliest citation of germline transmission: J:157516
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Allele Type:    Targeted (Not Specified) Mutations:    Insertion, Nucleotide substitutions   Mutation details: A loxP was inserted upstream of exon 3 and a STOP cassette followed by a floxed neo cassette and modified genomic sequence for exon 3 through 6. The modified exon 4 contains a nucleotide substitution that results in the amino acid substitution of arginine for lysine at position 225 (K225R). This amino acid is responsible for the ubiquitination-mediated degradation of the peptide. Cre mediated recombination removes the wild-type exon 3 through 6 and the neo cassette allowing for expression of a transcript containing the nucleotide substitution. (J:157516)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any H2-Ab1 Mutation:	81 strains or lines available

Original:	J:157516 Ohmura-Hoshino M, et al., Cutting edge: requirement of MARCH-I-mediated MHC II ubiquitination for the maintenance of conventional dendritic cells. J Immunol. 2009 Dec 1;183(11):6893-7
All:	7 reference(s)